Attemps to identify species-diagnostic RAPD markers useful in differentiation rye from wheat

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Laurentian University , Sudbury, Ont
StatementJacey J. Bennett
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Thus these assays could not prove to be of general use Two rye genome-specific random amplified polymorphic DNA (RAPD) markers were identified for detection of rye introgression in wheat.

The genetic diversity of rye cultivars by 5 RAPD markers was evaluated. Five primers gave 42 polymorphic fragments ( %) with an average of bands per primer. rice, spring wheat, winter. In this work we searched for genetic similarities in twelve wild rye species and subspecies and a control (S.

cereale ssp cereale. cv Walet). using RAPD and Ant) markers Ant, is useful for. In the present study, attempts Attemps to identify species-diagnostic RAPD markers useful in differentiation rye from wheat book made to identify species - diagnostic markers for six eucalypt species (E.

camaldulensis Dehnh, E. citriodora Hook, E. grandis W. Hill ex Maiden, E. pellita F. The feasibility of identifying inter-simple sequence repeat markers associated with seed weight in hexaploid wheat was tested The feasibility of identifying inter-simple sequence repeat markers.

Leaf rust resistance was estimated in 83 common wheat accessions carrying alien genetic material from the collection of the Vavilov Institute.

Eight accessions with seedling resistance and 27 accessions with adult plant resistance were found. Analysis with molecular markers revealed genes highly and moderately efficient in Russia—Lr24, Lr39, Lr21, and Lr37—and a rye translocation 1AL.1RS.

Details Attemps to identify species-diagnostic RAPD markers useful in differentiation rye from wheat FB2

Rye (Secale L.) is a member of family Poaceae (tribe Triticeae) and includes perennial or annual, self-incompatible or self-compatible, and cultivated, weedy or wild species.

Classification of the genus Secale is inconsistent, and comprises 3–4 to 8 species from the phylogenetic studies in the last ten years.

Progress in rye breeding has been significantly reduced due to involving a small. Although dominant markers segregating have been used for the development of an integrated map instead of two separated maps in different species including olive (Atienza et al.,Chafari et al.,De Keyser et al.,Wu et al., ), we were unable to obtain an integrated map of sufficient quality and thus these markers were.

Subsequently, PCR-based DNA markers, such as randomly amplified polymorphic DNA (RAPD) markers, were developed and used for diversity analysis, phylogenetic analysis, and for the identification of a taxonomic relationship among the members of genus Lens (Sharma et al., ; Ford et al., ; Ferguson et al., ), for linkage map.

Introduction. Rye (Secale cereale L.) is a member of the Triticeae tribe that evolved in the Fertile Crescent and adjacent regions analogously to its relatives wheat andbarley, and wheat separated within the past 11 million years and rye and wheat diverged 7 million years ago (Martis et al., ).In the regions of origin wild relatives of rye are found still today, esp.

Background The concept of marker-assisted selection. The concept of marker-assisted selection (MAS) was suggested 25 years ago by C. Smith and P. Simpson () and by Soller and Beckmann (; see more details in the next section).The idea was that: “It is unlikely that many of the polymorphisms identified by the new laboratory techniques will be the QTL themselves.

Brunell MS, Whitkus R, Lukaszewski AJ () Development of arm specific RAPD markers for rye chromosome 2R in wheat. Crop Sci – CrossRef Google Scholar Bullrich L, Tranquilli G, Pfluger LA, Suarezm EY, Barneix AJ () Bread-making quality and yield performance of 1BL/1RS wheat isogenic lines.

Aegilops geniculata Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat (Triticum aestivum) improvement through distant hybridization. In this study, the wheat–Ae.

geniculata alien disomic substitution line W was obtained from the BC1F8 progeny of a cross between the common wheat ‘Chinese Spring’ (CS) and Ae. geniculata Roth (serial. The initial attempts to identify olive cultivars in commercial olive oil samples using SSR markers were reported by Pasqualone et al.

and Breton et al. [25, 65]. The SSR profiles of olive oil DNA were identical to those obtained from leaves and drupes, showing the potential of SSR marker to be used for forensic applications.

Abstract. Rye (Secale spp.) is a member of the Triticeae tribe in the grass family, Poaceae (syn.

Description Attemps to identify species-diagnostic RAPD markers useful in differentiation rye from wheat EPUB

Gramineae), which also includes wheat (Triticum ssp.) and barley (Hordeum ssp.).Secale is a small but important cereal genus that includes perennial or annual, self-incompatible or self-compatible, and cultivated rye (S.

cereale L.), weedy rye, and several wild species. Collections from wheat infected wheat, rye, barley, and 48 different grass species. Collections from 12 other grass species did not infect wheat.

Stripe rust occurred as far east as the Black Hills in South Dakota, as far north as Duncan in British Columbia, and as far south as Mexico City (94). During the past decade, numerous studies have been published on molecular mapping of Fusarium head blight (FHB) resistance in wheat.

We summarize the relevant findings from 52 quantitative trait loci. Rye (Secale cereale L.) is a cereal crop of major importance in many parts of Europe and rye breeders are presently very concerned with the restrict pool of rye genetic resources narrowing of rye genetic diversity results from the presence of “Petkus” pool in most modern rye varieties as well as “Petkus” × “Carsten” heterotic pool in hybrid rye breeding programs.

The largest set of genotypes and markers was analysed by McCartney et al. () who reported allele sizes of 41 SSR markers on 79 wheat lines. The same marker haplotype as ‘Sumai 3’ for the SSR markers Xgwm, Xbarc and Xgwm (spanning Fhb1), was found in seven genotypes, including the highly resistant varieties ‘Ning Available from Physical-Mapping-Technologies ISBN Schwarzacher T, Ali N, Chaudhary HK, Graybosch R, Kapalande HV, Kinski E, Heslop-Harrison JS.

Fluorescent in situ hybridization as a genetic technology to analyzing chromosomal organization of alien wheat recombinant lines. pp in IAEA-TECDOC Physical Mapping Technologies for the Identification and. Rye (Secale cereale L.) is a cereal crop of major importance in many parts of Europe and rye breeders are presently very concerned with the restrict pool of rye genetic resources available.

Such narrowing of rye genetic diversity results from the presence of “Petkus” pool in most modern rye varieties as well as “Petkus” × “Carsten” heterotic pool in hybrid rye breeding programs.

De novo assembly. Trinity used an average of % of the ConDeTri cleaned reads in its assemblies and produced an average of 17, contigs. The mean contig lengths ranged from bp to bp (Table 1) and each of the accessions had contigs exceeding bp (Fig.

1).Additionally, Bowtie2 mapped ~ 68% of each accession’s contigs back to their raw reads (Additional file 1). RAPD analysis of wheat genotypes carrying stem rust resistance gene for identification of a marker.

In: DAE-BRNS Life Sciences Symposium on Molecular Biology of Stress Response and its Applications, BARC, Mumbai, India. DecemberBook of Abstracts, p. Hu XY, Ohm HW & Dweikat I Identification of RAPD markers linked to the gene Pm1 for resistance to powdery mildew in wheat.

Theoretical and Applied Genetics Theoretical and Applied Genetics Five levels of nitrogen (0, 46, 69, 92 and kg ha-1) and phosphorous (0, 10, 20, 30 and 40 kg ha-1) were used to evaluate productivity of wheat in response to NP fertilizers and to identify the most suitable NP rates for production of wheat of wheat under participatory approach by using Farmers Research Group (FRG).

Gudin, in Reference Module in Life Sciences, Interspecific crosses. As stated earlier, interspecific hybridization, either spontaneous or artificial, is at the origin of today׳s the 10 original wild species that have constituted the artificial species R.

hybrida, most are diploid, but some are tetraploid or modern rose is mostly represented by tetraploid. Marker-assisted Selection: Practice Selection Schemes for Marker-assisted Selection Bottlenecks in Application of Marker-assisted Selection Reducing Costs and Increasing Scale and Efficiency Traits Most Suitable for MAS Marker-assisted Gene Introgression Marker-assisted Gene Pyramiding Marker-assisted Hybrid.

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Paran and Michelmore () recommended that RAPD markers are converted to specific SCAR markers. RAPD-SCAR markers has been successfully used in. Middle Eastern on wheat and barley, and American on maize. Over the past few thousand years, more than half of the suitable land on Earth and virtually all of the most fertile land, has been converted for agricultural use.

Agriculture today is a global business, and a necessity for the production of food, drinks and other vital commodities such. Factors affecting this methodology include the nature of culture media used to optimize the recovery of strains (for greater depth see Volume 1, Chapter 1), the effects of physical factors such as temperature on the recovery of soil populations, besides the use of modern approaches including the application of monoclonal antibodies and of.

In vitro haploid production is among the new technologies that show great promise toward the goal of increasing crop yields by making similar germplasm available for many crops that was used to implement one of the greatest plant breeding success stories of this century, i.

e., the development of hybrid maize by crosses of inbred lines. Random amplified polymorphic DNA (RAPD) fingerprints of Pelargonium.

DNA aliquots from 13 cultivars of Pelargonium hortorum (lanes a to m), nine cultivars of P. peltatum (lanes n to v), and one individual of the wild subspecies P.

peltatum ssp. dibrachya (lane w) were amplified with the arbitrary mer primer OPG RAPD products were separated on a % agarose gel and stained with .To evaluate the current status of U.S.

winter wheat resistance to stem rust, validate markers associated with known genes, and identify new loci effective against the disease, an association mapping population was assembled with U.S.

winter wheat cultivars and breeding lines from the regional nurseries.